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Others will know better, however I think you want the skimmer in the middle section. And you may want/need to add another baffle/wall to the last chamber to reduce the chance of bubbles from the skimmer getting to your return pump.
Aerobic bacteria stains are good to convert ammonia to nitrate, and other strains of aerobic bacteria to nitrate. Then you can, if you chose, benefit from anaerobic bacteria that can survive deep inside dense live rock or a deep sand bed, to take nitrogen all the way out the tank, by converting nitrate to nitrogen gas which escapes the tank. Deep sand beds are dangerous because they could theoretically release a large bolus of toxic nitrogen or sulphur some say that will kill everything in your tank.
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Aerobic bacteria stains are good to convert ammonia to nitrate, and other strains of aerobic bacteria to nitrate. Then you can, if you chose, benefit from anaerobic bacteria that can survive deep inside dense live rock or a deep sand bed, to take nitrogen all the way out the tank, by converting nitrate to nitrogen gas which escapes the tank. Deep sand beds are dangerous because they could theoretically release a large bolus of toxic nitrogen or sulphur some say that will kill everything in your tank.
Sent from my VS986 using Tapatalk